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maxsep magnetic separator

ultramag magnetic separator - passofinto.fr

2021-7-13 · maxsep magnetic separator. MagneticSeparatorsSpherotech. Themagneticpegs ofUltraMagSeparatorfit between the wells underneath the 96 well plate The microplate is read in an appropriate microtiter plate reader by loading the microplate with the attachedUltraMagSeparatorinto the reader The magnets ensure that themagneticparticles stay

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Efficient clinical-scale enrichment of lymphocytes for use ...

2009-1-1 · As reported here and by collaborators , monocyte depletion can be achieved by incubating PBMC with Dynabeads, non-porous polystyrene superparamagnetic beads, in the absence of specific antibodies, by capture of adherent cells and removal via a large-scale MaxSep or ClinExVivo ™ magnetic separator. The MaxSep ‘semi-closed’ method requires ...

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A Phase I Trial of CD3/CD28-activated T Cells

2021-12-27 · moved by processing over a MaxSep Magnetic Separator (Nex-ell Therapeutics, Irvine, CA). Next, the T cells were activated and culture-expandedex vivo in a closed system in an adaptation of a process described previously (10). Briefly, the T cells were costimulated with Xcyte-Dynabeads (Xcyte Therapies, Seattle,

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Adoptive transfer of costimulated T cells induces ...

2003-9-15 · The magnetic beads were removed using a Baxter Fenwal Maxsep magnetic cell separation system. After cell culture, harvest was performed on the day of scheduled cell reinfusion with the Baxter Fenwal cell harvester; the cells were washed and resuspended in Plasmalyte A containing 1% HSA.

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Purging in BCR-ABL-positive acute lymphoblastic leukemia ...

Dynabeads (Dynal, Hamburg, Germany) and the Baxter MaxSep Magnetic cell separator (Baxter, Deerfield, IL, USA) were used for IMB purging in all patients. From 1992 to

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Expression of a Functional c-k/t Receptor on a Subset of ...

Cells were next adhered to a MaxSep Magnetic Cell Separator (Baxter HealthCare Corp., Deerfield, IL) for 10 min. Nonadherent cells were then labeled with directly conjugated mAbs and sorted for CD56 bright, CD56 dim, CD56 bright c-kit receptor-positive

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[18F]FHBG PET/CT Imaging of CD34-TK75 Transduced

2015-6-1 · After removing the Dynabeads using a Fenwell MaxSep Magnetic Separator (Baxter, Deerfield, IL), cells were washed in PBS containing 2 mmol/l ethylenediaminetetraacetic acid and 25% human serum albumin, then centrifuged (510 × g, 7 minutes) to concentrate them.

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Safety, tumor trafficking and immunogenicity of chimeric ...

2017-3-21 · On Day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL, USA) and resuspended in AIM-V medium with IL-2 (200 IU/mL). Transduction with CC49-ζ retroviral supernatant was performed on Days 5 and 7 in AIM-V medium containing IL-2 and polybrene (Aldrich, St Louis, MO, USA) using spinoculation at 800

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Magnetic Bead Cell Separation - ProImmune - Mastering ...

Magnetic bead sorting with R-PE- or biotin-labeled Pro5® Pentamers is a simple solution for applications requiring enrichment or depletion of antigen-specific T cells. If used as a pre-enrichment step before flow cytometry sorting, it will reduce both the number of cells that will have to be sorted and the required sorting time.

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Baxter Maxsep Fenwal magnetic cell separator 4r4511 | eBay

Baxter Maxsep Fenwal magnetic cell separator 4r4511. Condition is Used. Shipped with USPS Priority Mail or UPS Ground

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ultramag magnetic separator - passofinto.fr

2021-7-13 · maxsep magnetic separator. MagneticSeparatorsSpherotech. Themagneticpegs ofUltraMagSeparatorfit between the wells underneath the 96 well plate The microplate is read in an appropriate microtiter plate reader by loading the microplate with the attachedUltraMagSeparatorinto the reader The magnets ensure that themagneticparticles stay

Read More
A Phase I Trial of CD3/CD28-activated T Cells

2021-12-27 · moved by processing over a MaxSep Magnetic Separator (Nex-ell Therapeutics, Irvine, CA). Next, the T cells were activated and culture-expandedex vivo in a closed system in an adaptation of a process described previously (10). Briefly, the T cells were costimulated with Xcyte-Dynabeads (Xcyte Therapies, Seattle,

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Combined positive and negative cell selection from ...

2001-5-4 · Using these magnetic bead separation systems, two groups of sequential +/− selection (Isolex 300i version 1/MaxSep and Isolex 300i version 2/MaxSep) and

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Purging in BCR-ABL-positive acute lymphoblastic leukemia ...

Dynabeads (Dynal, Hamburg, Germany) and the Baxter MaxSep Magnetic cell separator (Baxter, Deerfield, IL, USA) were used for IMB purging in all patients. From 1992 to 1996, a triple cocktail of ...

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Expression of a Functional c-k/t Receptor on a Subset of ...

Cells were next adhered to a MaxSep Magnetic Cell Separator (Baxter HealthCare Corp., Deerfield, IL) for 10 min. Nonadherent cells were then labeled with directly conjugated mAbs and sorted for CD56 bright, CD56 dim, CD56 bright c-kit receptor-positive

Read More
Adoptive transfer of costimulated T cells induces ...

2003-9-15 · The magnetic beads were removed using a Baxter Fenwal Maxsep magnetic cell separation system. After cell culture, harvest was performed on the day of scheduled cell reinfusion with the Baxter Fenwal cell harvester; the cells were washed and resuspended in Plasmalyte A containing 1% HSA.

Read More
[18F]FHBG PET/CT Imaging of CD34-TK75 Transduced

2015-6-1 · After removing the Dynabeads using a Fenwell MaxSep Magnetic Separator (Baxter, Deerfield, IL), cells were washed in PBS containing 2 mmol/l ethylenediaminetetraacetic acid and 25% human serum albumin, then centrifuged (510 × g, 7 minutes) to concentrate them.

Read More
Prolonged survival and tissue trafficking following ...

2000-8-1 · On day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL) and resuspended in AIM-V medium with IL-2 and ritonavir. Transduction with CD4ζ retroviral supernatant at a multiplicity of infection of 2 viral particles per cell was performed on days 5 and 7 in AIM-V medium containing IL-2, ritonavir, and ...

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Safety, tumor trafficking and immunogenicity of chimeric ...

2017-3-21 · On Day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL, USA) and resuspended in AIM-V medium with IL-2 (200 IU/mL). Transduction with CC49-ζ retroviral supernatant was performed on Days 5 and 7 in AIM-V medium containing IL-2 and polybrene (Aldrich, St Louis, MO, USA) using spinoculation at 800

Read More
Adoptive transfer of costimulated T cells induces ...

2003-9-15 · The magnetic beads were removed using a Baxter Fenwal Maxsep magnetic cell separation system. After cell culture, harvest was performed on the day of scheduled cell reinfusion with the Baxter Fenwal cell harvester; the cells were washed and resuspended in Plasmalyte A containing 1% HSA.

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Rapid immune recovery and graft-versus-host disease-like ...

2009-6-9 · The beads were removed with a Baxter Fenwal Maxsep® magnetic separator, washed and concentrated with the Baxter Fenwal Harvester System, and resuspended in 100–500 mL 1:1 Plasmalyte A/Dextrose 5%, 0.45% NaCl containing 0.5–1% human serum albumin. The harvested cells were transported by courier from the cell production facility to the ...

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Baxter Maxsep Fenwal magnetic cell separator 4r4511 | eBay

Baxter Maxsep Fenwal magnetic cell separator 4r4511. Condition is Used. Shipped with USPS Priority Mail or UPS Ground

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The Effects of Anti-CD3/CD28 Coated Beads and IL-2 on ...

Removal of CD3/CD28 beads from the cell suspension was accomplished by passing cells plus beads (3-30 x 10(9) cells in 2-12 L) over a MaxSep magnetic separator using gravity-driven flow.

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Expression of a Functional c-k/t Receptor on a Subset of ...

Cells were next adhered to a MaxSep Magnetic Cell Separator (Baxter HealthCare Corp., Deerfield, IL) for 10 min. Nonadherent cells were then labeled with directly conjugated mAbs and sorted for CD56 bright, CD56 dim, CD56 bright c-kit receptor-positive

Read More
Prolonged survival and tissue trafficking following ...

2000-8-1 · On day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL) and resuspended in AIM-V medium with IL-2 and ritonavir. Transduction with CD4ζ retroviral supernatant at a multiplicity of infection of 2 viral particles per cell was performed on days 5 and 7 in AIM-V medium containing IL-2, ritonavir, and ...

Read More
Safety, tumor trafficking and immunogenicity of chimeric ...

On Day 3, the beads were removed using a Maxsep magnetic bead separator (Baxter, Roundlake, IL, USA) and resuspended in AIM-V medium with IL-2 (200 IU/mL). Transduction with CC49-ζ retroviral supernatant was performed on Days 5 and 7 in AIM-V medium containing IL-2 and polybrene (Aldrich, St Louis, MO, USA) using spinoculation at 800 x g for ...

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Efficient clinical-scale enrichment of lymphocytes for use ...

Clinical-scale lymphocyte enrichment from a leukapheresis product has been performed most routinely using costly magnetic bead separation systems that deplete monocytes, but this procedure may leave behind residual beads or antibodies in the enriched cell product. Counterflow centrifugal elutriation has been demonstrated previously to enrich monocytes

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NEXELL THERAPEUTICS INC - Quarterly Report (10-Q)

1999-8-16 · B. "Distributed Products" shall bear the meaning set forth in Schedule 1.2(B) attached hereto. C. "Field of Distribution" means the worldwide market in connection with the Maxsep(R) and/or Isolex(R) Products used for the selection of cells with one or more of the CD34, B Cell, T Cell or breast cancer antibodies for the treatment, mitigation, prophylaxis or selection of/for

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Shanghai LIVIC Filtration System Co., Ltd.: Hydraulics ...

Discover all the products from Shanghai LIVIC Filtration System Co., Ltd. and see a list of their distributors. Contact the manufacturer directly for a quote.

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المزيد من المعلومات

الصين -تشنغ تشو -المنطقة الوطنية للتنمية الصناعية للتكنولوجيا المتطورة، جادة العلوم رقم 169.

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